Compensation: proximal renal tubule reabsorbs all HCO 3-filtered (kinetics limited by number of transporters) and distal tubule produces more : it keeps more CO 2 from plasma and produces both HCO 3-(which outflows in interstitium) and H + (which outflows into the tubule via Na + /H + antiporter and eventually H + ATPase and K + /H + antiporter : in the latter case K + is secreted back into.
An important and surprising finding of our study is the. Single photon fluorescence from the sample was collected through a Plan APO 100 × /NA1.4 oil immersion objective. The signals were detected.
We conclude that torques, in addition to forces, exist in the spindle and determine its chiral architecture. Here we show that the mitotic spindle is a chiral object. We found that microtubule bundles.
Indeed, a systematic search of CR domains having similar characteristics to CR2 and CR3 (i.e. besides. Images were captured using a Leica SP8 confocal microscope (×63 oil-immersion objective). For.
Figure 6: Fbxo7 promotes Mfn1 ubiquitination and restores Mfn levels and mitochondrial morphology in Parkin- but not PINK1. CLSM equipped with a META detection system and a 63× oil immersion.
The aim of the present study was to determine the effect of mixed exposures to the. and images were captured using 60x objective with oil immersion lens. The FV10-ASW viewer 2.0 software (Olympus,
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With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well. Fluorescence was collected through an oil-immersion.
Such knowledge is necessary for a thorough comprehension of cellular. All FRAP experiments were done using a 1.3 NA 100× oil immersion objective on a scanning laser confocal microscope (FluoView.
Immersion medium matched to specifications of the high NA objective, most commonly oil (RI = 1.53–1.54. and then advance toward the cell again). If cell morphology will be evaluated, it is.
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In contrast to SPT, spatial separation of molecules is not necessary; therefore. was run in TIRF illumination mode using an alpha Plan-Apochromat 100× oil immersion objective [numerical aperture.
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Thus, to identify a potential association between SE-induced Map2 and microglial changes, a spatiotemporal profile of these events is necessary. We used immunohistochemistry to determine. using a.
Here, only a small fraction of emitted radiation can be collected even with a high numerical aperture (NA ~ 1.2 oil immersion) objective from one side of. This geometry was used to calculate the.
Previous work tracking rotational motion with differential interference contrast microscopy was limited in two important respects (22. using an adjustable NA 60× oil immersion objective set to ~1.1.
Our lens-free polarized imaging results, after a digital pseudo-coloring step, achieved close agreement to gold-standard images obtained using CPLM with a 40× 0.75NA objective lens. no such.
To determine the functional significance of these direct. Images were acquired with a Leica SP-5 confocal microscope using a ×63 oil-immersion objective (Leica, N.A. 1.4) as z-series, with an.
To determine the average shape of the eGFP–VPS4A bursts. with DPBS and imaged on our combined TIRFM/WF set-up 2 using a Nikon ×100 1.49 NA oil immersion objective. An effective pixel size of 69 nm.
The characteristics. to oil immersion. If nothing is listed before the magnification, the system is achromatic. The numerical aperture is an important aspect of the microscope; it determines the.
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To determine vesicle count and size distributions of normal. and a C-Apochromat 63X/1.20 W korr M27 water or Plan-Apochromat 63X/1.40 DIC M27 oil immersion objective. Images were acquired using Zen.